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96-well high-affinity binding microplate costar assay plate  (Corning Life Sciences)

 
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    Corning Life Sciences 96-well high-affinity binding microplate costar assay plate
    96 Well High Affinity Binding Microplate Costar Assay Plate, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/96-well+high-affinity+binding+microplate/96+well+high+affinity+binding+microplate+costar+assay+plate/pm40172224-164-2-8
    Average 90 stars, based on 1 article reviews
    96-well high-affinity binding microplate costar assay plate - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: Treatment of women affected with polycystic ovary syndrome
    Article Snippet: A 96-well high-affinity binding microplate (Corning) was coated with 50 μl of capture antibody (monoclonal antibody, anti-bovine LH beta subunit, 518B7; University of California, Lillian Sibley UC Davis) at a final dilution of 1:1000 (in Na2CO3/NaHCO3, 0.1M, pH 9.6) and incubated overnight at 4° C. Wells were incubated with 200 μl of blocking buffer (5% (w/v) skim milk powder in 1×PBS-T (0.01M PBS, 1.09 g of Na2HPO4 (anhydrous), 0.32 g of NaH2PO4 (anhydrous) and 9g NaCl in 1 L distilled water with 0.05% Tween 20 (pH 7.4, Sigma #P9416)) for 2 hr at RT.

    Article Title: Perinatal exposure to known endocrine disrupters alters ovarian development and systemic steroid hormone profile in rats.
    Article Snippet: Briefly, a 96-well high-affinity binding microplate (Corning Life Sciences, Corning, NY) was coated overnight with 50 μl of the monoclonal antibody, anti-bovine LHβ subunit (518B7, provided by L. Sibley, University of California, UC Davis) at a dilution of 1:1000 (in 0.015 M Na2CO3 and 0.035 M NaCO3 coating buffer, pH 9.6).

    Article Title: The preoptic Kisspeptin/nNOS/GnRH (KiNG) neuronal network regulates rhythmic LH release through a dual activation-inhibition mechanism
    Article Snippet: Briefly, we used a 96-well high-affinity binding microplate (Corning) coated with 50 μL of primary capture antibody (bovine LHβ subunit, 518B7; L. Sibley; University of California, UC Davis) diluted at 1μg/ml in 0.01M PBS.

    Article Title: Dicer ablation in Kiss1 neurons impairs puberty and fertility preferentially in female mice
    Article Snippet: A 96-well high-affinity binding microplate (Corning) was coated with 50 μl of the monoclonal antibody, anti-bovine LHβ subunit, 518B7 (L. Sibley; University of California, UC Davis) at a dilution of 1:1000 (in PBS: Na 2 HPO 4 /NaH 2 PO 4 /NaCl 0.1 M, pH 7.4) and incubated overnight at 4 °C.

    Article Title: Preventing and correcting polycystic ovary syndrome by targeting anti-Müllerian hormone signaling in minipuberty and adulthood in mice.
    Article Snippet: For this study, we used an ultrasensitive ELISA method to measure circulating LH levels in the whole blood in mice as validated previously to assess pulsatile LH release in femalemice over the estrous cycle.37,38 Briefly, this ELISA uses a 96-well high-affinity binding microplate (Corning) coatedwith 50 mL of primary capture antibody (bovine LHb subunit, 518B7; L. Sibley; University of California, UC Davis) at a dilution of 1:1000.

    Article Title: Overactivation of GnRH neurons is sufficient to trigger polycystic ovary syndrome-like traits in female mice.
    Article Snippet: Plasma supernatant was transferred to sterile 200-μL Eppendorf tubes and stored at −80 ◦C.

    Article Title: Female sexual behavior is disrupted in a preclinical mouse model of PCOS via an attenuated hypothalamic nitric oxide pathway
    Article Snippet: Briefly, we used a 96-well high-affinity binding microplate (Corning) coated with 50 μL of primary capture antibody (bovine LHβ subunit, 518B7; L. Sibley; University of California, UC Davis) at a dilution of 1:1,000.

    Article Title: Overactivation of GnRH neurons is sufficient to trigger polycystic ovary syndrome-like traits in female mice
    Article Snippet: For this study, we used an ultrasensitive ELISA method to measure circulating LH levels in the whole blood in mice as validated previously to assess pulsatile LH release in female mice over the estrous cycle., Briefly, this ELISA uses a 96-well high-affinity binding microplate (Corning) coated with 50 μL of primary capture antibody (bovine LHβ subunit, 518B7; L. Sibley; University of California, UC Davis) at a dilution of 1:1000.

    Binding Assay:

    Article Title: Treatment of women affected with polycystic ovary syndrome
    Article Snippet: A 96-well high-affinity binding microplate (Corning) was coated with 50 μl of capture antibody (monoclonal antibody, anti-bovine LH beta subunit, 518B7; University of California, Lillian Sibley UC Davis) at a final dilution of 1:1000 (in Na2CO3/NaHCO3, 0.1M, pH 9.6) and incubated overnight at 4° C. Wells were incubated with 200 μl of blocking buffer (5% (w/v) skim milk powder in 1×PBS-T (0.01M PBS, 1.09 g of Na2HPO4 (anhydrous), 0.32 g of NaH2PO4 (anhydrous) and 9g NaCl in 1 L distilled water with 0.05% Tween 20 (pH 7.4, Sigma #P9416)) for 2 hr at RT.

    Article Title: Perinatal exposure to known endocrine disrupters alters ovarian development and systemic steroid hormone profile in rats.
    Article Snippet: Briefly, a 96-well high-affinity binding microplate (Corning Life Sciences, Corning, NY) was coated overnight with 50 μl of the monoclonal antibody, anti-bovine LHβ subunit (518B7, provided by L. Sibley, University of California, UC Davis) at a dilution of 1:1000 (in 0.015 M Na2CO3 and 0.035 M NaCO3 coating buffer, pH 9.6).

    Article Title: The preoptic Kisspeptin/nNOS/GnRH (KiNG) neuronal network regulates rhythmic LH release through a dual activation-inhibition mechanism
    Article Snippet: Briefly, we used a 96-well high-affinity binding microplate (Corning) coated with 50 μL of primary capture antibody (bovine LHβ subunit, 518B7; L. Sibley; University of California, UC Davis) diluted at 1μg/ml in 0.01M PBS.

    Article Title: Dicer ablation in Kiss1 neurons impairs puberty and fertility preferentially in female mice
    Article Snippet: A 96-well high-affinity binding microplate (Corning) was coated with 50 μl of the monoclonal antibody, anti-bovine LHβ subunit, 518B7 (L. Sibley; University of California, UC Davis) at a dilution of 1:1000 (in PBS: Na 2 HPO 4 /NaH 2 PO 4 /NaCl 0.1 M, pH 7.4) and incubated overnight at 4 °C.

    Article Title: Preventing and correcting polycystic ovary syndrome by targeting anti-Müllerian hormone signaling in minipuberty and adulthood in mice.
    Article Snippet: For this study, we used an ultrasensitive ELISA method to measure circulating LH levels in the whole blood in mice as validated previously to assess pulsatile LH release in femalemice over the estrous cycle.37,38 Briefly, this ELISA uses a 96-well high-affinity binding microplate (Corning) coatedwith 50 mL of primary capture antibody (bovine LHb subunit, 518B7; L. Sibley; University of California, UC Davis) at a dilution of 1:1000.

    Article Title: Overactivation of GnRH neurons is sufficient to trigger polycystic ovary syndrome-like traits in female mice.
    Article Snippet: Plasma supernatant was transferred to sterile 200-μL Eppendorf tubes and stored at −80 ◦C.

    Article Title: Female sexual behavior is disrupted in a preclinical mouse model of PCOS via an attenuated hypothalamic nitric oxide pathway
    Article Snippet: Briefly, we used a 96-well high-affinity binding microplate (Corning) coated with 50 μL of primary capture antibody (bovine LHβ subunit, 518B7; L. Sibley; University of California, UC Davis) at a dilution of 1:1,000.

    Article Title: Overactivation of GnRH neurons is sufficient to trigger polycystic ovary syndrome-like traits in female mice
    Article Snippet: For this study, we used an ultrasensitive ELISA method to measure circulating LH levels in the whole blood in mice as validated previously to assess pulsatile LH release in female mice over the estrous cycle., Briefly, this ELISA uses a 96-well high-affinity binding microplate (Corning) coated with 50 μL of primary capture antibody (bovine LHβ subunit, 518B7; L. Sibley; University of California, UC Davis) at a dilution of 1:1000.

    Incubation:

    Article Title: Treatment of women affected with polycystic ovary syndrome
    Article Snippet: A 96-well high-affinity binding microplate (Corning) was coated with 50 μl of capture antibody (monoclonal antibody, anti-bovine LH beta subunit, 518B7; University of California, Lillian Sibley UC Davis) at a final dilution of 1:1000 (in Na2CO3/NaHCO3, 0.1M, pH 9.6) and incubated overnight at 4° C. Wells were incubated with 200 μl of blocking buffer (5% (w/v) skim milk powder in 1×PBS-T (0.01M PBS, 1.09 g of Na2HPO4 (anhydrous), 0.32 g of NaH2PO4 (anhydrous) and 9g NaCl in 1 L distilled water with 0.05% Tween 20 (pH 7.4, Sigma #P9416)) for 2 hr at RT.

    Article Title: Perinatal exposure to known endocrine disrupters alters ovarian development and systemic steroid hormone profile in rats.
    Article Snippet: Briefly, a 96-well high-affinity binding microplate (Corning Life Sciences, Corning, NY) was coated overnight with 50 μl of the monoclonal antibody, anti-bovine LHβ subunit (518B7, provided by L. Sibley, University of California, UC Davis) at a dilution of 1:1000 (in 0.015 M Na2CO3 and 0.035 M NaCO3 coating buffer, pH 9.6).

    Article Title: The preoptic Kisspeptin/nNOS/GnRH (KiNG) neuronal network regulates rhythmic LH release through a dual activation-inhibition mechanism
    Article Snippet: Briefly, we used a 96-well high-affinity binding microplate (Corning) coated with 50 μL of primary capture antibody (bovine LHβ subunit, 518B7; L. Sibley; University of California, UC Davis) diluted at 1μg/ml in 0.01M PBS.

    Article Title: Dicer ablation in Kiss1 neurons impairs puberty and fertility preferentially in female mice
    Article Snippet: A 96-well high-affinity binding microplate (Corning) was coated with 50 μl of the monoclonal antibody, anti-bovine LHβ subunit, 518B7 (L. Sibley; University of California, UC Davis) at a dilution of 1:1000 (in PBS: Na 2 HPO 4 /NaH 2 PO 4 /NaCl 0.1 M, pH 7.4) and incubated overnight at 4 °C.

    Article Title: Preventing and correcting polycystic ovary syndrome by targeting anti-Müllerian hormone signaling in minipuberty and adulthood in mice.
    Article Snippet: For this study, we used an ultrasensitive ELISA method to measure circulating LH levels in the whole blood in mice as validated previously to assess pulsatile LH release in femalemice over the estrous cycle.37,38 Briefly, this ELISA uses a 96-well high-affinity binding microplate (Corning) coatedwith 50 mL of primary capture antibody (bovine LHb subunit, 518B7; L. Sibley; University of California, UC Davis) at a dilution of 1:1000.

    Article Title: Overactivation of GnRH neurons is sufficient to trigger polycystic ovary syndrome-like traits in female mice.
    Article Snippet: Plasma supernatant was transferred to sterile 200-μL Eppendorf tubes and stored at −80 ◦C.

    Article Title: Female sexual behavior is disrupted in a preclinical mouse model of PCOS via an attenuated hypothalamic nitric oxide pathway
    Article Snippet: Briefly, we used a 96-well high-affinity binding microplate (Corning) coated with 50 μL of primary capture antibody (bovine LHβ subunit, 518B7; L. Sibley; University of California, UC Davis) at a dilution of 1:1,000.

    Article Title: Overactivation of GnRH neurons is sufficient to trigger polycystic ovary syndrome-like traits in female mice
    Article Snippet: For this study, we used an ultrasensitive ELISA method to measure circulating LH levels in the whole blood in mice as validated previously to assess pulsatile LH release in female mice over the estrous cycle., Briefly, this ELISA uses a 96-well high-affinity binding microplate (Corning) coated with 50 μL of primary capture antibody (bovine LHβ subunit, 518B7; L. Sibley; University of California, UC Davis) at a dilution of 1:1000.

    Blocking Assay:

    Article Title: Treatment of women affected with polycystic ovary syndrome
    Article Snippet: A 96-well high-affinity binding microplate (Corning) was coated with 50 μl of capture antibody (monoclonal antibody, anti-bovine LH beta subunit, 518B7; University of California, Lillian Sibley UC Davis) at a final dilution of 1:1000 (in Na2CO3/NaHCO3, 0.1M, pH 9.6) and incubated overnight at 4° C. Wells were incubated with 200 μl of blocking buffer (5% (w/v) skim milk powder in 1×PBS-T (0.01M PBS, 1.09 g of Na2HPO4 (anhydrous), 0.32 g of NaH2PO4 (anhydrous) and 9g NaCl in 1 L distilled water with 0.05% Tween 20 (pH 7.4, Sigma #P9416)) for 2 hr at RT.

    Article Title: Perinatal exposure to known endocrine disrupters alters ovarian development and systemic steroid hormone profile in rats.
    Article Snippet: Briefly, a 96-well high-affinity binding microplate (Corning Life Sciences, Corning, NY) was coated overnight with 50 μl of the monoclonal antibody, anti-bovine LHβ subunit (518B7, provided by L. Sibley, University of California, UC Davis) at a dilution of 1:1000 (in 0.015 M Na2CO3 and 0.035 M NaCO3 coating buffer, pH 9.6).

    Article Title: The preoptic Kisspeptin/nNOS/GnRH (KiNG) neuronal network regulates rhythmic LH release through a dual activation-inhibition mechanism
    Article Snippet: Briefly, we used a 96-well high-affinity binding microplate (Corning) coated with 50 μL of primary capture antibody (bovine LHβ subunit, 518B7; L. Sibley; University of California, UC Davis) diluted at 1μg/ml in 0.01M PBS.

    Article Title: Dicer ablation in Kiss1 neurons impairs puberty and fertility preferentially in female mice
    Article Snippet: A 96-well high-affinity binding microplate (Corning) was coated with 50 μl of the monoclonal antibody, anti-bovine LHβ subunit, 518B7 (L. Sibley; University of California, UC Davis) at a dilution of 1:1000 (in PBS: Na 2 HPO 4 /NaH 2 PO 4 /NaCl 0.1 M, pH 7.4) and incubated overnight at 4 °C.

    Article Title: Preventing and correcting polycystic ovary syndrome by targeting anti-Müllerian hormone signaling in minipuberty and adulthood in mice.
    Article Snippet: For this study, we used an ultrasensitive ELISA method to measure circulating LH levels in the whole blood in mice as validated previously to assess pulsatile LH release in femalemice over the estrous cycle.37,38 Briefly, this ELISA uses a 96-well high-affinity binding microplate (Corning) coatedwith 50 mL of primary capture antibody (bovine LHb subunit, 518B7; L. Sibley; University of California, UC Davis) at a dilution of 1:1000.

    Article Title: Overactivation of GnRH neurons is sufficient to trigger polycystic ovary syndrome-like traits in female mice.
    Article Snippet: Plasma supernatant was transferred to sterile 200-μL Eppendorf tubes and stored at −80 ◦C.

    Article Title: Female sexual behavior is disrupted in a preclinical mouse model of PCOS via an attenuated hypothalamic nitric oxide pathway
    Article Snippet: Briefly, we used a 96-well high-affinity binding microplate (Corning) coated with 50 μL of primary capture antibody (bovine LHβ subunit, 518B7; L. Sibley; University of California, UC Davis) at a dilution of 1:1,000.

    Article Title: Overactivation of GnRH neurons is sufficient to trigger polycystic ovary syndrome-like traits in female mice
    Article Snippet: For this study, we used an ultrasensitive ELISA method to measure circulating LH levels in the whole blood in mice as validated previously to assess pulsatile LH release in female mice over the estrous cycle., Briefly, this ELISA uses a 96-well high-affinity binding microplate (Corning) coated with 50 μL of primary capture antibody (bovine LHβ subunit, 518B7; L. Sibley; University of California, UC Davis) at a dilution of 1:1000.



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